HYDROGEN-ION CONCENTRATION RELATIONS IN A THREE-SALT SOLUTION

Hydrogen ion concentration changes in mitochondrial membranes.

1. Bromthymol blue added to rat liver mitochondria at concentrations in the range 0.4 to 4.0 mpmoles per mg of protein is tightly bound as indicated by centrifugation and washing of the mitochondria. 2. The absorbance of the dye is increased on adding Ca++ or Mn++ to the mitochondria in the absence of a permeant anion. The extent of increase is 0.03 cm-l for 167 PM Ca++ at 618 rnp for 0.5 mpmol...

Colorimetric Determination of the Hydrogen Ion Concentration in Small Quantities of Solution.*

The hydrogen ion concentration (1) of many solutions and of culture media is readily determined by means of buffer solutions and suitable indicators. The usual procedure, in determining calorimetrically the hydrogen ion concentration, requires a considerable volume of solution. Frequently the quantity of solution available in experiments upon plants or animals is extremely limited in amount. It...

Hydrogen Ion Concentration and Pepsin Digestion

One of the most striking peculiarities of enzyme action is the fact that the activity of the enzyme is limited to a definite range of acidity. If the solution is more or less acid than this the enzyme is practically inactive. SSrensen 1 showed that for a number of enzymes the determiuing factor was the hydrogen ion concentration and not the total acidity of the solution. In attempting to accoun...

The Hydrogen-Ion Concentration of Cholera Stools

Design of Hydrogen Ion Concentration Measurement System

This paper presents an application for measuring the concentration of hydrogen ion by using ion selective electrodes. By this research we hope to provide useful information and verify the feasibility of measuring the concentration of other ions in the same way for sensing sweat. In this research, a pH electrode is used to generate an electrical potential which reflects the concentration of hydr...